2005) were identified using the ARDRA technique, though their identity was not confirmed by sequencing. Mycoplasma species isolated from cats (Criado-Fornelio et al. It is thus suggested that the prediction power of ARDRA clusters in identifying clone phylogeny be regarded with caution.Īttempts to use ARDRA to identify species within particular genera have only been partially successful. Although ARDRA-based clusters separated clones into different genera, these phylogenetic clusters did not overlap with trees constructed according to sequence alignment, calling into question the intra-genus ARDRA-based phylogeny. ARDRA was performed in silico on 48,759 sequences from the Ribosomal Database Project, and it was found that the fragmentation profiles were not necessarily unique for each sequence in the database, resulting in different species sharing fragmentation profiles. Here we used ARDRA as a model to examine the capacity of restriction-based techniques for clone identification, and the possibility of deriving phylogenetic information from ARDRA-based dendrograms. But if you choose not to provide it, FEMA may delay or deny assistance.Amplified ribosomal DNA restriction analysis (ARDRA) and restriction fragment length polymorphism were originally used for strain typing and for screening clone libraries to identify phylogenetic clusters within a microbial community. You don’t have to provide the requested information.
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